Coding

Part:BBa_K2160000:Design

Designed by: Emma Finlayson-Trick   Group: iGEM16_Dalhousie_Halifax_NS   (2016-10-14)


Beta-1,4-D-glycosidic bonds in cellulose -> cellulobiose


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Carefully designed primers containing restriction enzyme cut sites so that none of the sites were found in the endoglucanase nucleotide sequence or the biobrick vector.


Source

Ruminiclostridium thermocellum nucleotide sequence

References